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  3. Sample size

Check sample size

Estimate how many animals you need per group. The effect size, alpha and power values shown are the usual defaults; adjust them to your study.

Student's t-test

Quantitative analysis with two groups.

ANOVA

Quantitative analysis with more than two groups.

Binomial test

Proportions (yes/no outcomes), comparing against a 50% baseline.

Relative change from the 50% baseline you want to detect.
Formulas are the same as in the original Mouse Randomization app (normal-approximation sample-size equations). Read the theoretical considerations for the meaning of significance level, power and effect size.